Journal: Cell reports

Article Title: Regulation of serine/threonine kinase Akt Activation by NAD + -dependent deacetylase SIRT7

doi: 10.1016/j.celrep.2017.01.009

Figure Lengend Snippet: (A) MCF7 cells were transfected with negative siRNA or SIRT7 siRNA and were then treated with the indicated drugs for 72 hours. Cell survival was determined by MTS assay as described in Methods.

Article Snippet: For deacetylation assay, 1 μg of acetylated FKBP51 was incubated with 500 ng of SIRT7 protein (TP305658, Origene), or immuno-purified WT or HY mutant Flag-SIRT7, in the deacetylation buffer (50 mM Tris-HCL [pH 9.0]), 4 mM MgCl2, 0.2 mM DTT, 1 μg/ml bepstatin, 1 μg/ml leupeptin, 1 μg/ml pepstatin and 1mM NAD + ) for 30 min.

Techniques: Transfection, MTS Assay